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Western blot analysis of various Aspergillus proteins, using mouse polyclonal sera against rAsp f 2 and rabbit sera against the p40 cytosolic component of A. fumigatus. Lanes: a, molecular weight markers; b to e, treated with anti-rAsp f 2 sera; f to i, treated with anti-p40 sera; b and f, A. fumigatus culture filtrate antigens (AF102); c and g, CFC fraction from A. fumigatus; d and h, rAsp f 2; e and i, ASPND1 from A. nidulans.
