FIG. 7.

(A) Specific binding of laminin to Asp f 2. rAsp f 1, rAsp f 12, rAsp f 2, nAsp f 2, and crude A. fumigatus culture filtrates (100 μg/well) were incubated with 10 μg of laminin per ml. The subsequent incubation was with a 1:1,000 dilution of mouse antilaminin antibody. The ELISA IgG absorbance was measured at 490 nm (for details, see Materials and Methods). (B) Dose-dependent binding of laminin (1 ng to 10 μg) to solid-phase coated Asp f 2. Open bars, IgG absorbance of laminin-antilaminin interaction on Asp f 2-coated wells; closed bars, absorbance values for direct laminin-antilaminin binding on laminin-coated wells.