TABLE 1.

Asp f 2 concentration and pH of A. fumigatus culture (AF104) at various time intervals

Incubation time	Mycelial wt (g)	pH of culture medium	Asp f 2 concn (1,000 ng/ml of crude extract)a
			Culture filtrate	Mycelia
0 h		7.25
12 h	0.276	7.2	10
24 h	0.723	6.9	16	11
48 h	3.3	6.4	8	69
72 h	3.7	5.7	12	185
96 h	5.8	5.9	9	200
5 days	6.32	5.7	7	281
7 days	8.4	5.65	9	403
14 days	4.9	4.5	9	284
21 days	3.7	4.35	5	291

^aCalculated from the Asp f 2 standard curve by ELISA, using concentrations of 10 to 500 ng of Asp f 2/ml versus anti-Asp f 2 antibody.