TABLE 3.
Mean numbers of MLN cells and proportions of CD4+ and CD8+ T cells obtained from IL-10-deficient mice, wild-type mice, and heterozygous mice kept in either SPF or germfree conditionsa
| Mouse group | No. of MLN cells (106 [no. of mice]) | % CD4+ T cells (no. of mice) | % CD8+ T cells (median)b (no. of mice) | CD44 level (median)bc (no. of mice) |
|---|---|---|---|---|
| SPF wild type | 22.7 ± 2.2 (29) | 36.6 ± 2.2 (15) | 15.4 ± 0.8 (15.3) (15) | 88.6 ± 5.99 (82.5) (12) |
| SPF IL-10 deficient | 33.4 ± 4.0* (25) | 28.4 ± 1.8* (12) | 22.8 ± 1.9 (24.1)** (12) | 130.3 ± 10.14 (127.5)** (14) |
| Germfree heterozygote | 9.5 ± 2.1 (6) | 36.7 ± 2.1 (7) | 21.4 ± 1.7 (21.1) (7) | 101 ± 4.56 (100) (7) |
| Germfree IL-10 deficient | 8.9 ± 1.2 (12) | 35.1 ± 1.6 (10) | 18.4 ± 1.6 (17.9) (10) | 97.5 ± 2.23 (98) (11) |
a
Except where indicated values are means ± standard errors of the mean. *, significantly different mean value compared to those for SPF wild-type, germfree heterozygote, and germfree IL-10-deficient mice (P < 0.05); **, significantly different median value compared to that for SPF wild-type mice (P < 0.05).
b
Median values were used for statistical comparisons because of nonparametric data.
c
Fluorescence intensity units.