FIG. 1.

Effects of agr and sar on invasion. A dose response invasion assay was performed by infecting MAC-T cell monolayers for 2 h with increasing numbers of S. aureus cells so that the multiplicity of infection (MOI) was altered within the ranges indicated. The monolayers were then washed, and incubation of cocultures proceeded in the presence of gentamicin to kill extracellular bacteria. Strains RN6390 (wild type [wt]), RN6911 (agr), ALC136 (sar), and ALC135 (agr sar) were used. Error bars represent the means ± the standard errors of the means.