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Relative expression of CAPN3 mRNA by quantitative polymerase chain reaction. Relative expression of CAPN3 mRNA revealed a decreased mutated CAPN3 transcript level in the proband, their younger sister and mother, while the proband's father showed normal expression of wild-type CAPN3 mRNA. The comparative threshold cycle (2-ΔΔCq) method was used to determine the relative expression levels of the wild-type and mutated CAPN3 mRNA. CAPN3, calpain 3.
