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. 1998 Nov;66(11):5252–5259. doi: 10.1128/iai.66.11.5252-5259.1998

FIG. 3.

FIG. 3

Wild-type and mutant holotoxins in native-PAGE immunoblots with stacking and resolving gels (indicated at the left) developed with anti-Stx1A serum (A) or an anti-Stx1B monoclonal antibody (B). Lanes: 1, Stx1B; 2, wild-type Stx1A; 3, wild-type Stx1A associated with Stx1B; 4, G234E Stx1A; 5, G234E Stx1A plus Stx1B; 6, A231D Stx1A; 7, A231D Stx1A plus Stx1B; 8, F226Y Stx1A; 9, F226Y Stx1A plus Stx1B; 10, A231D-G234E Stx1A; 11, A231D-G234E Stx1A plus Stx1B. The positions of the holotoxin and Stx1B and Stx1A monomers are indicated at the right.