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Interaction of wild-type Stx1A and A231D-G234E Stx1A with synthetic lipid vesicles. Purified wild-type (•) or A231D-G234E (■) A subunits at 18 μg/ml were combined with increasing concentrations of 70% DOPC–30% DOPG (mol/mol) vesicles. Fluorescence intensities were obtained at an excitation wavelength of 290 nm and an emmission wavelength of 323 nm and expressed as a percentage of that of the toxin without lipid vesicles. The results of one representative experiment are shown.
