FIG. 5.

Cytochalasin D and nocodazole but not wortmannin inhibit invasion of HBMEC by L. monocytogenes. HBMEC were infected with L. monocytogenes EGD in the presence or absence of different inhibitors. The inhibitors cytochalasin D, nocodazole, and wortmannin were dissolved in dimethyl sulfoxide. The monolayers were pretreated with the inhibitors for 45 min, and the infection was performed as described in the text. Lanes: A, without inhibitor; B and C, nocodazole (10 and 20 μM); D and E, cytochalasin D (0.1 and 1 μM); F to I, wortmannin (5, 10, 50, and 100 nM). ∗, P < 0.01 versus no inhibitor; ∗∗, P < 0.001 versus no inhibitor.