Fig 6.

HSV-1 gG modifies the protein composition of EV. Liquid chromatography-mass spectrometry was performed on VRF obtained from vector- or gG-transduced ARPE-19 cells (five biological replicates per condition). (A) Volcano plot displaying up-regulated (red) and down-regulated (green) proteins determined using student´s t-test with a significance cutoff of 15% FDR. (B) Heatmap showing the names of genes whose protein products were increased in VRF of gG-expressing ARPE-19 cells compared to vector-transduced cells. The color code represents the raw z-scores. (C and D) Immunoblots detecting galectin-1 and GAPDH in VRF (C) and whole-cell lysates (WCL) (D) obtained from stably transduced ARPE-19. Data are presented as mean ± standard deviation of the mean. ** P < 0.01 (unpaired t-test with Welch’s correction)