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. 2024 Jan 9;15(2):e02749-23. doi: 10.1128/mbio.02749-23

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Copyright © 2024 Lv et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Fig 7 — Analysis of the location of Golgi-tracker green and STX 6 in the mature spore and sporoplasm of N. bombycis. (A) Analysis of the location of Golgi-tracker green and STX 6 in the mature spore of N. bombycis. Golgi-tracker green was used for fluorescence staining analysis of the mature spore. Immunofluorescence analysis treated the mature spores with rabbit STX 6 polyclonal antibody and negative rabbit serum, respectively. (B) Analysis of the location of Golgi-tracker green and STX 6 in the sporoplasm of N. bombycis. Golgi-tracker green was used for fluorescence staining analysis of the sporoplasm. Immunofluorescence analysis treated the sporoplasms with rabbit STX 6 polyclonal antibody and negative rabbit serum, respectively. The nuclei were stained with DAPI (blue), and the membrane was stained with DiI (red). The white triangles and arrows indicate the polaroplast in the mature spores and sporoplasm, respectively. The white dotted line indicates the position of spore wall and the polar tube. Bar, 2 µm.