Fig 4.

Strains lacking OmpU have increased VPS production. (A) Representative VPS dot-blot showing serial dilutions of VPS samples obtained from biofilms of indicated strains. Biofilms were grown for 24 h at 30°C on LB agar plates, and VPS was isolated as described in the Materials and Methods. The immunoblot was probed with VPS-specific antibodies. (B) Relative VPS amounts were calculated based on VPS immunoblots. The experiment was performed with three biological replicates and two technical replicates per each biological replicate. Immunoblots were analyzed using ImageJ-Fiji software, and statistical significance was determined using Student’s t-test. ***P ˂ 0.0001. Error bars represent the standard error of the mean. (C) Colony corrugation phenotypes of strains with indicated genotypes after 72 h of growth at 30°C. Images are representative of two biological replicates, with three technical replicates per biological replicate. Scale bars = 1 mm.