| Antibodies |
|
| Mouse anti-mCherry |
Living Colors |
632543; RRID:AB_2307319 |
| Rabbit anti-cFos |
Synaptic Systems |
226 008; RRID:AB_2891278 |
| Alexa 555 Donkey anti-mouse |
Invitrogen |
A-31570; RRID: AB_2536180 |
| Alexa 488 Donkey anti-rabbit |
Invitrogen |
A-21206; RRID:AB_2535792 |
|
| Bacterial and virus strains |
|
| AAVrg- pmSyn1-EBFP-Cre |
Hongkui Zeng (Madisen et al.)56
|
Addgene 51507-AAVrg |
| AAV9-EF1a-double floxed-hChR2(H132R)-mCherry-WPRE-HGHpa |
Karl Deisseroth |
Addgene 20297-AAV9 |
| AAV9-hSyn-Flex GCaMP6f-WPRE-SV40 |
Douglas Kim and GENIE Project (Chen et al.)57
|
Addgene 100833-AAV9 |
| AAV8-hSyn-DIO mCherry |
Bryan Roth |
Addgene 50459-AAV8 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Carprofen injectable solution |
Santa Cruz Biotechnology, Inc. |
sc-395914Rx |
| Vectashield mounting medium |
VectorLabs |
H-1000-10 |
| Jet liquid clear |
Lang Dental |
N/A |
| Jet denture repair powder |
Lang Dental |
N/A |
|
| Critical commercial assays |
|
| RNAscope Multiplex Fluorescent Reagent Kit v2 |
ACDBio |
323270 |
| RNAscope mCherry probe |
ACDBio |
431201 |
| RNAscope Rn-Slc17a6-C2 probe |
ACDBio |
317011-C2 |
| RNAscope Rn-Gad1-C3 probe |
ACDBio |
316401-C3 |
|
| Deposited data |
|
| Processed data for statistical analysis |
Current paper |
https://doi.org/10.5281/zenodo.10059759
|
|
| Experimental models: Organisms/strains |
|
| Long Evans (Blue Spruce) outbred rats |
Envigo/Inotiv |
HsdBlu:LE |
|
| Software and algorithms |
|
| MATLAB |
Mathworks |
RRID:SCR_001622 |
| R |
The R Project |
https://cran.r-project.org/mirrors.html
|
| Python |
Python |
https://www.python.org/downloads/
|
| Synapse software |
Tucker-Davis Technologies |
https://www.tdt.com/component/synapse-software/
|
| HALO Image Analysis Platform |
Indica Labs |
RRID:SCR_018350 |
| ImageJ |
Schneider et al.58
|
https://ImageJ.net/ij/download.html
|
| Behavioral event encoding model |
Parker et al.59
|
https://github.com/nfparker/event_encoding_model
|
| airPLS algorithm |
Zhang et al.60
|
https://github.com/zmzhang/airPLS
|
| Fiber photometry analysis code |
Current paper |
https://doi.org/10.5281/zenodo.10366545
|
| Optogenetics analysis code |
Current paper |
https://doi.org/10.5281/zenodo.10366408
|
| Statistical analysis code |
Current paper |
https://doi.org/10.5281/zenodo.10059759
|
| Gramm |
Morel61
|
https://github.com/piermorel/gramm
|
|
| Other |
|
| Fiberoptic cannula for optogenetics |
RWD Life Science Inc. |
R-FOC-BF300C39NA |
| Fiberoptic cannula for fiber photometry |
Doric Lenses |
MFC_300/330–0.37_9mm_MF2.5_FLT |
| Mono fiberoptic patchcords |
Doric Lenses |
MFP_400/460/900–0.48_1m_FCM-MF1.25 |
| Fiber photometry workstations |
Tucker-Davis Technologies |
RZ5P |
| Newport photoreceivers |
Doric Lenses |
NPM_2151_FOA_FC |
| Fluorescence Minicubes |
Doric Lenses |
FMC4_AE(405)_E(460–490)_F(500–550)_S |
| LED driver |
Thor Labs |
DC4104 |
| Connectorized LD: 405 nm |
Doric Lenses |
CLED_405 |
| Connectorized LD: 465 nm |
Doric Lenses |
CLED_465 |
| Confocal microscope |
Nikon |
AX R |
| Deconvolution microscope |
Nikon |
Ti-E |
| Dual small animal stereotaxic instrument |
David Kopf Instrumental |
Model 942 |
| Cryostat |
Leica Biosystems |
CM1900 |
