Fig 1.

wPip does not restrict DENV-2 replication in Aag2 cells. Ae. aegypti-derived cell lines (Aag2) stably infected with wMel, wAlbB, and wPip strains were infected with DENV-2 at MOI 1 and compared to their matched Wolbachia-free line. Infectious virus and cellular RNA were collected from each cell line 7 days post-infection for analysis by tissue culture infectious dose ₅₀ (TCID₅₀) and qRT-PCR. The numbers in parentheses represent the average number of Wolbachia per cell (Wolbachia 16S rRNA/RPS17), determined in parallel wells for each independent experiment prior to DENV-2 inoculation. (A) Data are the median number of virus genome copies relative to the RPS17 mosquito housekeeping gene ±IQR. (B) Data are the median infectious viral titres ± IQR determined by TCID₅₀. Data are derived from at least 3 (A) or 2 (B) independent experiments performed with triplicate biological replicates each. Statistical analyses were performed using Mann-Whitney test, where **P < 0.005, ****P < 0.0001.