Skip to main content
. 2024 Jan 5;170(1):001424. doi: 10.1099/mic.0.001424

Table 2.

Primers used in this study

Primers

Sequence (5′−3′)

Purpose

Reference

SXTint-F

GCTGGATAGGTTAAGGGCGG

PCR and sequencing

[49]

SXTint-R

CTCTATGGGCACTGTCCACATTG

PCR and sequencing

[49]

FloRfl-F

CGGAATTCCCATCCTGCGGGAGCG

PCR, cloning and sequencing

This study

FloRfl-R

AACTGCAGTCCAGCGCTTTCACAC

PCR, cloning and sequencing

This study

FloR-F

CCGGAATTCGTCATGACCACCACACGC

Cloning and sequencing

This study

FloRNΔ29-F

CCGGAATTCGCGATGGATATTTATCTCCCTG

Cloning and sequencing

This study

FloRCΔ36-R

AAAACTGCAGCTAGTCACCGTTTAAAAGTG

Cloning and sequencing

This study

PBAD-F

TCGCTAACCAAACCGGTAAC

Sequencing

[47]

PBAD-R

GATGCCTGGCAGTTCCCTAC

Sequencing

[47]

SXTjun-F

ATAAAGTCAAGATCAGCGAAAAT

PCR and sequencing

This study

Prfcint-R

AGAGTCAACTGCGGTCAGAG

PCR and sequencing

[49]

FloRcΔ12-R

AAAACTGCAGCTACCGAAGGAGCACCAGCCCC

Cloning and sequencing

This study

FloRnΔ4-F

CCGGAATTCACCATGCGCCCCGCGTGGGCCTATAC

Cloning and sequencing

This study

FloRP1-F

CCGGAATTCGTAGCAATTCATATTC

Cloning and sequencing

This study

FloRP2-F

CCGGAATTCTCATCTGATTGCTGACG

Cloning and sequencing

This study

SXTstrA-F

GCGTGACCGCCTCATTTGG

PCR

This study

SXTstrA-R

CCCGTGCATTGAAGAGTTTTAG

PCR

This study

SXTstrB- F

ATGTTGCTCGAATATGCCGG

PCR

This study

SXTstrB- R

GCCGGATCGTAGAACATATTGG

PCR

This study

SIint-F

ACATCCATTTTCATAAT

PCR

This study

SIint-R

GTGCATTTGGATACTTT

PCR

This study

SIjun-F1

ATTAAAGCTGGTCAATAC

PCR

This study

SIjun-R1

GAAGCTTACCTTCATTGGAT

PCR

This study

SIjun-F2

ATACAAAAAAGCTTCAGTTC

PCR

This study

SIjun-R2

GCTGCGCTGCTTCTAACT

PCR

This study

StrAB-F

CCGGAATTCCCATTGAATCGAACT

Cloning and sequencing

This study

StrAB-R

AAAACTGCAGGTCGCTTG

Cloning and sequencing

This study

StrA-R1

AAAACTGCAGGAACATCAACCCCAAGT

Cloning and sequencing

This study

StrB-F1

CCGGAATTCTTGATGTTCATGCCG

Cloning and sequencing

This study

StrA-F2

ATTGCTAACGCCGAAGAG

RT-PCR

This study

StrB-R2

CCGCGCAGTTCATCAGCAAT

RT-PCR

This study

StrAcΔ6-R

AAAACTGCAGCTACAATCGCAGATAGAAGG

Cloning and sequencing

This study

StrAnΔ27-F

CCGGAATTCGTTATGTTTCGACGTGGTGACG

Cloning and sequencing

This study

FloRrtm-F

TGTCGCGGTCGGTATTGTC

qRT-PCR

This study

FloRrtm-R

CGTCGAACTCTGCCAAAGC

qRT-PCR

This study

StrArtm-F

GGGCAGCGCCAGATGA

qRT-PCR

This study

StrArtm-R

GCTTCGATCCCCAATACATTG

qRT-PCR

This study

M13-F

GTAAAACGACGGCCAGT

Sequencing

NEB

M13-R

AACAGCTATGACCATG

Sequencing

NEB

recA-F

GCAATTTGGTAAAGGCTCCA

qRT-PCR

[27]

recA-R

GTTGTGCAGCAGCAATCAGT

qRT-PCR

[27]