Droplet composition and RH impact on GAS airborne longevity. CELEBS measurements of GAS emm1 survival to compare the effect of (a) direct loading of THY broth culture into CELEBS (control) or following centrifugation and suspension in fresh THY broth prior to levitation (resuspended) for 20 min at 50 % RH; (b) droplet composition in LB broth, THY broth, MEM 2 % FBS, and artificial saliva after 5 and 20 min of levitation at 50 % RH; (c) differing RH in LB broth or MEM 2 % FBS, at 30, 50 and 75 % RH after 20 min of levitation; (d) artificial saliva in differing RH, at 30, 50 and 75 % RH after 5 min of levitation. Each datapoint shows the % viability from a single levitation. The central line shows the mean % viability and the error bars show the standard deviation. Significance determined by Student’s t-test, where *P<0.05.