FIG. 4.

Specificity of the PAFR/BC- or PAFR/CN-mediated inhibition of Salmonella invasion of BHK cell transfectants. Shown are effects of transfecting the PAFR/BC (BC) or PAFR/CN (CN) cDNA on the invasion of Salmonella (open bars), Listeria (filled bars), E. coli expressing invasin (hatched bars), and EPEC (gray bars). Cells were transfected by electroporation, and invasion assays were performed as described for Fig. 2B at an MOI of 10. Data presented are based on the percentage of bacterium-specific invasion observed in cells transfected with an equal dose of the PAFR cDNA. Data represents the mean ± standard error of the mean from three separate transfections assayed simultaneously and repeated at least twice with similar results. Statistical significance was determined by Student’s paired t test. ∗, P < 0.05 versus PAFR transfectants.