Figure 1.

Epigenetic disorder increases across the murine lifespan. (A) Diagram of the approach for measuring regional disorder (RD). (B) Density of all genomic regions assessed with respect to their Spearman correlation coefficients between RD and age. (C) The relationship between global disorder and age in mice. (D) Average RD across all regions that gain disorder with age (correlation coefficient ≥0.25), or (E) lose disorder with age (correlation coefficient ≤−0.25. (F) Manhattan plot of the distribution of FDR corrected p-values of the relationship between RD and age. Red line marks a commonly used genome wide significance value of p = 5 × 10⁻⁸. Enrichment of age associated RD in genes (G), promoters (H), enhancers (I), PRC2 target genes (J), transcription factor binding sites (K), CTCF binding sites (L), and average CpG density (M). (N) The six most significant gene ontology biological processes (GO:BP) for regions gaining or losing disorder with age. Regions which gain disorder with age are shown in blue and regions which lose disorder with age are shown in red.