Figure 3.

Epigenetic disorder underlies epigenetic clock signals. (A) Distribution of Petkovich epigenetic clock sites (red) across correlation coefficients between regional disorder (RD) and age. (B) Average absolute correlation coefficient between RD and age of regions which are included in the Petkovich epigenetic clock (red) compared to those which are not included. (C) Error of epigenetic age estimates produced by leave-one-out cross validation (LOOCV) for each data type. (D–G) Manhattan plots showing the robustness for each region (i.e., the proportion of clocks each region was selected in) across (D) CpG methylation (black), (E) regional methylation (RM; yellow), (F) regional entropy (RE; light blue), and (G) RD (dark blue) contexts. (H–K) Density plots showing the distribution of clock sites for each data type across correlation coefficients between RD and age. (L) Overlap between regions included in epigenetic clocks produced from each data type. (M) Relationship between delta epigenetic age (chronological age – predicted age) and age-adjusted global disorder for each data type.