Figure 5.

Epigenetic disorder during de-differentiation and development. (A) Epigenetic age predictions using each of the representative epigenetic clocks and (B) global disorder of kidney fibroblasts (black), kidney derived iPSCs (grey), lung fibroblasts (dark purple), and lung derived iPSCs (pink). Plot shows median, upper and lower quartiles, maximum, and minimum. Outliers beyond 1.5 interquartile range are plotted. (C) Distribution of regions which gain (blue) or lose (red) disorder after de-differentiation across correlation coefficients between regional disorder (RD) and age. (D) Effect sizes of de-differentiation on the RD epigenetic clock. Stubbs CpG methylation (E), RM (F), RE (G), and RD (H) epigenetic clock predictions of samples during embryonic development. (I) Global disorder of samples during embryonic development. (J) distribution of regions which gain (blue) or lose (red) disorder during early development across correlation coefficients between regional disorder (RD) and age. (K) Effect sizes of development on the RD epigenetic clock.