FIG. 3.

Triton X-114 partitioning assays indicated that Ncp29 and Ncp35 are membrane associated. Parasite lysates were separated with Triton X-114 into an aqueous phase (lanes A) that contains soluble proteins and a detergent phase (lanes D) that contains membrane-associated proteins. Western blot analysis with MAb 6C11 (α-p29) indicated that Ncp29 partitioned exclusively with the detergent phase. Western blot analysis with MAb 5H5 (α-p35) indicated that Ncp35 partitioned primarily with the detergent phase, although a minor portion was found in the aqueous phase.