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Immuno-EM localization of Ncp29 and Ncp35 in extracellular tachyzoites of N. caninum. Both Ncp29 (MAb 6C11 (A) and Ncp35 (MAb 5H5) (B) were localized to the parasite cell membrane but were not found in micronemes, dense granules, or rhoptries. Occasional gold particles were found in the cytoplasm within small vesicles. No labeling of the parasites was observed with MAb DG52 against T. gondii SAG1 (C). Bars, approximately 0.2 μm.
