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. 2024 Feb 2;20(2):e1011999. doi: 10.1371/journal.ppat.1011999

Fig 7. ANP32A and ANP32B bridge HBV RNA-ELAVL1-CRM1 and mediate HBV RNAs nuclear export.

Fig 7

(A-D) HepG2-NTCP cells were transfected with ANP32A or ANP32B targeted siRNA. The cells were infected with HBV at an MOI = 200 and were maintained with DMEM medium supplemented with 2.5% DMSO for 5 days. (A) Levels of ANP32A, ANP32B, and HBc were determined by WB. (B) Levels of HBV DNA in supernatants were determined by qPCR (% of siCtrl). Secreted HBeAg and HBsAg levels were determined by ELISA (% of siCtrl). (C) Subcellular levels of HBV mRNA in cytoplasm and nucleus were determined by qPCR (% of siCtrl). Graphs were shown as mean ± SD. *, p < 0.05; **, P < 0.01; ***, p < 0.001. (D and E) ANP32A and (or) ANP32B knockdown HepAD38 cells were maintained in DMEM medium containing 2% DMSO for 2 days. The binding of (D) HBV RNA-CRM1 and (E) HBV RNA-ELAVL1 were detected by RIP assay. (F) Schematic description of the HBV RNA binding proteins complex.