Figure 1.

Experimental design. A: continuous model. Timeline of manipulations and measurements performed on mice that were maintained on customized trace (0.04%)- or normal/high (0.30%)-Na versions of Teklad 2920x soy-free diet. B: postwean model. Timeline of manipulations and measurements performed on mice that were treated with 0.04% or 0.30% Na diets from weaning [postnatal day (P)21] to 6 wk of age (P42), before return to standard (0.15% Na) 2920x diet. BIS, bioimpedance spectroscopy; BMR, basal metabolic rate; NMR, nuclear magnetic resonance. Figure created with BioRender.