Extended Data Fig. 7. AMPK-mediated p300 nucleocytoplasmic shuttling.
a, b, Phosphorylation of p300 (p-p300) at S89 in an AMPK-dependent manner. Blots are representative of three biologically independent experiments (N = 3). One-way ANOVA with post hoc Tukey test. c, p300 activity in control or AMPKα1/2 knockdown cells. N = 4. One sample t-test. d, Validation of AMPK WT and α1/α2 double knock-out (dKO) MEF using anti-total AMPKα and anti-phosphorylated AMPKα antibody. N = 3. e, Inhibition of p300 nuclear transport in starved AMPK dKO MEF. N = 3. One sample t-test. f, Failure of increased phosphorylation of p300 at S89 in AMPK dKO MEF by depleting AAs. N = 3. One sample t-test. g, Rescue of p300 shuttling in Glc-depleted AMPK dKO MEFs after reconstitution with myc-tagged AMPKα2, but not myc-tagged AMPK α2 K45R. The expression levels following transfection are depicted by the quantification of the myc-tag. N = 3. One-way ANOVA with post hoc Tukey test. h, Measurement of acetyltransferase activity in p300 WT, dNLS or S89A construct. N = 4. Two-tailed paired t test. i, mTORC1 activity in His-tagged p300 WT, SA or SD expressing p300 KO cells. The expression levels following transfection are depicted by the quantification of the His-tag. N = 3. One-way ANOVA with post hoc Tukey test. Data are presented as mean values +/- s.d. unless otherwise specified. Source numerical data and unprocessed blots are available in source data.