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. 2024 Jan 24;26(2):235–249. doi: 10.1038/s41556-023-01338-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a, Enhanced degradation of progerin protein by treatment with SEL or A485. Representative western blots of GFP-progerin inducible cell lines following different times of Dox removal. Blots are representative of three biologically independent experiments (N = 3). ** p < 0.01, *** p < 0.001 vs. vehicle (DMSO) treated cells (two-way ANOVA with Bonferroni post-test). b, Autophagy mediated progerin degradation by treatment with A485, 991 or SEL. N = 3. One-way ANOVA with post hoc Tukey test. c, More colocalisation of progerin with endogenous LC3-positive vesicles in HGPS fibroblasts after treatment with A485. N = 3, about 50 cells scored per condition per experiment. Two-tailed paired t-test. Scale bar, 5 μm, 2 μm (enlarged images). d, Regulation of progerin degradation in p300 KO expressing His-tagged p300 WT, dNLS or I265A (IA). N = 3. One-way ANOVA with post hoc Tukey test. Data are presented as mean values +/- s.d. unless otherwise specified. Source numerical data and unprocessed blots are available in source data.

Source data