TABLE 3.
Effects of metabolic inhibitors on P. gingivalis A7436 invasion of BAEC
| Inhibitor | Targeta | % Inhibitionb |
|---|---|---|
| Cytochalasin D | EC (actin) | 97.5 ± 1.2 |
| Nocodazole | EC (microtubule) | 87.4 ± 1.3 |
| Staurosporine | EC (protein kinase) | 86.6 ± 4.2 |
| Cycloheximide | EC (protein synthesis) | 2.5 ± 0.8 |
| Sodium azide | EC (energy metabolism) | 80.5 ± 2.1 |
| Sodium azide | P. gingivalis (energy metabolism) | 99.1 ± 0.1 |
| Chloramphenicol | P. gingivalis (protein synthesis) | 3.2 ± 0.4 |
| Rifampin | P. gingivalis (RNA synthesis) | 4.1 ± 1.9 |
| Nalidixic acid | P. gingivalis (DNA synthesis) | 3.3 ± 1.1 |
| Protease inhibitors | P. gingivalis (proteases) | 99.1 ± 0.2 |
a
Inhibitors were preincubated with either the endothelial cells or P. gingivalis as described in the text.
b
Reduction in invasion in the presence of inhibitors as compared with that of a control with no inhibitor present. Values are means ± standard deviations (n = 3).