Extended Data Fig. 3. Altered protein expression in TSCs in response to mTORi treatment.
(a) K-means clustering of differentially expressed proteins identified with MetaboAnalyst 4.0. Spearman’s rank correlation coefficient (ρ) and corresponding p value are shown in (A) and (B). (b) Representative images of H3K9me2 and Lamin B1 expression in normal and mTORi-treated ESCs and TSCs. Scale bar: 10 µm. (c) Quantifications of the H3K9me2 and Lamin B1 signal intensity along single-nucleus cross-sections of normal and mTORi-treated ESCs and TSCs (n = 15 cells, 48 h treatment). (d) Transmission electron microscopy images of cell-cell adhesion complexes and stress fibers. Structures are highlighted in yellow. Scale bar: 2 µm.