Extended Data Fig. 6. Detailed characterization of carnitine-supplemented embryos.
(a) Representative IF images of embryos stained against Ki67 and NANOG in each condition (n = 8 embryos/condition). Scale bar = 50 µm. (b) Quantification of DAPI-normalized Ki67 intensities in the embryo. n = number of cells, *: p < 0.05, one way ANOVA, Dunnett’s post hoc test. (c) Single embryo metabolomics quantification data per metabolite. Each dot represents an embryo. n = 4–12 embryos have been used for each measurement. (d) Number of OCT4-positive cells per embryo in each condition. n = number of embryos, *: p < 0.05, one way ANOVA, Dunnett’s post hoc test. (e) Number of TE cells per embryo in each condition. TE cells are defined as DAPI-positive and OCT4-negative. n = number of embryos, *: p < 0.05, one way ANOVA, Dunnett’s post hoc test. (f) Representative IF images of embryos stained against cleaved-CASPASE3 and OCT4 in each condition (n = 5 embryos/condition). As positive control, E4.5 embryos were exposed to UV with a UV crosslinker. energy 4000 uJ/cm2 for 8 seconds and subsequently cultured for another 6 hours. Across conditions, some TE cells display cleaved-caspase3 staining. Scale bar = 50 µm.