Extended Data Fig. 7. FOXO and CPT expression dynamics.
(a) FOXO1 and FOXO3 protein expression over time in ESCs and TSCs treated with mTORi. (b) Embryo survival curves in the shown conditions. n = number of embryos. Statistical test is the G-rho family test of Harrington and Fleming89. (c) Single embryo proteomics heatmap displaying the Pearson correlation between the individual samples using the expression values of the differentially expressed proteins. (d) IF staining for CPT1A and SOX2 in each condition. Embryos (n = 5/condition) were paused for 5 days with or without L-carnitine, FOXO11i and FOXO1i+carnitine. Representative embryos’ z-projections are shown. Scale bar = 50 µm. Additionally, quantifications of area normalized CPT1A, pULK and LC3B are shown. a and b: p < 0.05 compared to E4.5 and D5 mTORi, respectively, computed with a one way ANOVA and Tukey HSD posthoc test.