Fig. 4. l-carnitine facilitates lipid usage during developmental pausing.
a, Electron microscopy images of E4.5 and diapaused embryos. LDs are highlighted in yellow. Scale bar, 2 µm. b, Quantifications of LD abundance and size and the number of mitochondria in proximity to LDs. Whole-blastocyst electron microscopy images (single plane) from four individual E4.5 or diapaused embryos were used. ZOI, zone of interest. Data are presented as median ± standard deviation. Statistical test is a two-sided Student’s t-test. c, Visualization of LDs in E4.5 or diapaused embryos via BODIPY staining (green). Red channel shows an actin stain (CellMask). d, Number of LDs per embryo in the shown conditions. Live embryos were stained with BODIPY (LDs), CellMask (actin) and Hoechst (DNA). n is the number of embryos per condition. *P < 0.05, one-way ANOVA, Dunnett’s post hoc test. Data are presented as median ± standard deviation. e, Representative images of cells with one, two, three, four or greater than four LDs. LDs are shown in green, cell membrane in red and the nucleus in blue. Scale bar, 5 µm. f, LD area for each condition per category, that is, one, two, three, four and greater than four LDs per cell. n is the number of cells. Horizontal lines show median values.