Extended Data Fig. 1. Characterization of mTOR inhibition in ESCs and TSCs.
(a) Bright field images of ESCs and TSCs during normal proliferation, at D3 and D4 of pausing and upon release from mTORi. Scale bar: 100 µm. (b) Mean protein expression (pause and release vs control t = 0) of pluripotency genes in ESCs and stage-specific TE marker expression in TSCs using a previously curated gene list73. (C-E) Levels of phospho-mTOR (c), phospho-S6 (d), and phospho-AKT (e) in normal and mTORi-treated (48 hours) ESCs and TSCs. Single-cell, DAPI-normalized quantifications are shown on right panels. n = number of cells. Two biological replicates were performed.