Table 1.

PCR efficiency and linearity for pDNA and gDNA as calibrators

Target	Parameter	Calibrator	Mean	SD	Variance	p-value*
Lectin	Slope	pDNA	− 3.52	0.02	0.60 × 10–3	0.553
		gDNA	− 3.53	0.05	2.93 × 10–3
	PCR efficiency (%)	pDNA	92.25	0.88	0.77	0.592
		gDNA	91.91	1.97	3.87
SYHT0H2	Slope	pDNA	− 3.51	0.01	3.63 × 10–5	0.226
		gDNA	− 3.53	0.03	9.07 × 10–4
	PCR efficiency (%)	pDNA	92.83	0.22	0.05	0.225
		gDNA	91.93	1.06	1.13
MON87751	Slope	pDNA	− 3.46	0.01	0.61 × 10–4	0.884
		gDNA	− 3.47	0.06	3.90 × 10–3
	PCR efficiency (%)	pDNA	94.54	0.29	0.09	0.904
		gDNA	94.36	2.36	5.57
DAS-44406-6	Slope	pDNA	− 3.53	0.05	2.42 × 10–3	0.834
		gDNA	− 3.54	0.03	0.82 × 10–3
	PCR efficiency (%)	pDNA	92.07	1.73	2.99	0.824
		gDNA	91.80	1.01	1.02
DAS-81419-2	Slope	pDNA	− 3.47	0.01	0.72 × 10–4	0.395
		gDNA	− 3.51	0.07	5.49 × 10–3
	PCR efficiency (%)	pDNA	94.15	0.32	0.10	0.402
		gDNA	92.70	2.66	7.08

SD repeatability standard deviation

^*p-value was calculated at 95% confidence level, using the t-test