Table 2.
Repeatability for the pDNA and gDNA calibration curve
| Target | pDNA calibrator | gDNA calibrator | ||||||
|---|---|---|---|---|---|---|---|---|
| Copy no | Mean of Ct value | SD | RSD (%) | Copy no | Mean of Ct value | SD | RSD (%) | |
| Lectin | 900,000 | 19.51 | 0.37 | 1.92 | 88,496 | 23.16 | 0.20 | 0.87 |
| 90,000 | 23.03 | 0.38 | 1.63 | 8,849.6 | 26.61 | 0.27 | 1.02 | |
| 9000 | 26.60 | 0.40 | 1.50 | 884.96 | 30.14 | 0.28 | 0.94 | |
| 900 | 30.11 | 0.39 | 1.29 | 88.5 | 33.70 | 0.31 | 0.92 | |
| 90 | 33.58 | 0.39 | 1.17 | 8.85 | 37.28 | 0.38 | 1.03 | |
| SYHT0H2 | 900,000 | 19.68 | 0.21 | 1.06 | 88,496 | 23.33 | 0.12 | 0.52 |
| 90,000 | 23.17 | 0.17 | 0.73 | 8,849.6 | 26.86 | 0.10 | 0.36 | |
| 9000 | 26.79 | 0.20 | 0.74 | 884.96 | 30.31 | 0.11 | 0.35 | |
| 900 | 30.23 | 0.20 | 0.65 | 88.5 | 33.85 | 0.04 | 0.11 | |
| 90 | 33.69 | 0.17 | 0.52 | 8.85 | 37.49 | 0.26 | 0.68 | |
| MON87751 | 900,000 | 20.49 | 0.04 | 0.21 | 88,496 | 23.85 | 0.02 | 0.10 |
| 90,000 | 23.94 | 0.01 | 0.03 | 8,849.6 | 27.46 | 0.01 | 0.02 | |
| 9000 | 27.51 | 0.03 | 0.09 | 884.96 | 30.96 | 0.00 | 0.01 | |
| 900 | 30.96 | 0.02 | 0.07 | 88.5 | 34.38 | 0.08 | 0.24 | |
| 90 | 34.28 | 0.07 | 0.19 | 8.85 | 37.72 | 0.24 | 0.63 | |
| DAS-44406-6 | 900,000 | 18.66 | 0.18 | 0.99 | 88,496 | 22.39 | 0.07 | 0.31 |
| 90,000 | 22.17 | 0.14 | 0.65 | 8,849.6 | 25.83 | 0.05 | 0.18 | |
| 9000 | 25.77 | 0.09 | 0.35 | 884.96 | 29.33 | 0.03 | 0.11 | |
| 900 | 29.26 | 0.10 | 0.34 | 88.5 | 32.99 | 0.10 | 0.31 | |
| 90 | 32.76 | 0.07 | 0.22 | 8.85 | 36.49 | 0.07 | 0.20 | |
| DAS-81419-2 | 900,000 | 18.92 | 0.00 | 0.02 | 88,496 | 22.60 | 0.06 | 0.27 |
| 90,000 | 22.35 | 0.01 | 0.04 | 8,849.6 | 26.06 | 0.07 | 0.29 | |
| 9000 | 25.93 | 0.01 | 0.04 | 884.96 | 29.52 | 0.10 | 0.34 | |
| 900 | 29.33 | 0.01 | 0.04 | 88.5 | 33.06 | 0.11 | 0.33 | |
| 90 | 32.79 | 0.03 | 0.10 | 8.85 | 36.66 | 0.26 | 0.70 | |
SD repeatability standard deviation, RSD repeatability relative standard deviation