Table 4.
Quantification results of GM soybean events using pDNA and gDNA calibrators
| Target | Calibrator | Reference value (%) |
Trueness | Accuracy | Repeatability | |
|---|---|---|---|---|---|---|
| Mean of measured value |
Bias (%) | Recovery (%) | RSD (%) | |||
| SYHT0H2 | pDNA | 3.0 | 3.08 | 2.56 | 102.56 | 8.78 |
| 1.0 | 1.03 | 3.33 | 103.33 | 8.99 | ||
| gDNA | 3.0 | 2.93 | − 2.44 | 97.56 | 7.18 | |
| 1.0 | 1.00 | 0.33 | 100.33 | 6.48 | ||
| MON87751 | pDNA | 3.0 | 2.74 | − 8.67 | 91.33 | 4.48 |
| 1.0 | 0.89 | − 10.67 | 89.33 | 5.74 | ||
| gDNA | 3.0 | 2.83 | − 5.67 | 94.33 | 0.93 | |
| 1.0 | 0.97 | − 3.00 | 97.00 | 2.73 | ||
| DAS-44406-6 | pDNA | 3.0 | 3.55 | 18.22 | 118.22 | 11.38 |
| 1.0 | 1.18 | 18.00 | 118.00 | 3.69 | ||
| gDNA | 3.0 | 3.25 | 8.33 | 108.33 | 5.12 | |
| 1.0 | 1.16 | 16.33 | 116.33 | 7.99 | ||
| DAS-81419-2 | pDNA | 3.0 | 3.07 | 2.33 | 102.33 | 0.33 |
| 1.0 | 0.88 | − 11.67 | 88.33 | 2.36 | ||
| gDNA | 3.0 | 3.11 | 3.78 | 103.78 | 3.83 | |
| 1.0 | 0.95 | − 4.67 | 95.33 | 6.74 | ||