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. 2024 Feb 14;14:3746. doi: 10.1038/s41598-024-53947-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs of the immunohistochemical expression of leptin in periaortic fat showing: (a) positively stained thin rim of adipocytes’ cytoplasm. intense expression of leptin in various types of adipocytes, marked decreased leptin expression in ZnONPs treated groups. The positive brown color is quantified as optical density by image analysis software. Each bar represents the mean ± SE of 8 rats. *vs normal control group, ^@vs obese group, ^#vs ZnONPs (5 mg/kg) at p < 0.05. ZnONPs: Zinc oxide nanoparticle.