FIG. 8.

Preexisting inclusions do not require bacterial protein synthesis for fusion. HeLa cells were infected with C. trachomatis for 1 h at 37°C and incubated at 32°C for 24 h. Sample A was fixed and stained for C. trachomatis at this time. Samples B and C were further incubated at 37°C for 24 h in the absence (B) or presence (C) of CAM. The bacteria and host cells were visualized as described in the legend to Fig. 1. Each value represents the mean of three determinations (200 cells counted for three slides each), and error bars represent 1 standard deviation. Asterisks represent significant differences (P < 0.002) compared to the value for sample A.