Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1967 Oct;42(10):1334–1342. doi: 10.1104/pp.42.10.1334

Senescence: Action of Auxin and Kinetin in Control of RNA and Protein Synthesis in Subcellular Fractions of Bean Endocarp 1

Joseph A Sacher 1
PMCID: PMC1086728  PMID: 16656660

Abstract

A comparative study was made of the effects of auxin (α-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder pole beans (Phaseolus vulgaris, L.). Auxin caused considerable enhancement of incorporation of labeled precursors into RNA and protein of all subcellular fractions, and induced net synthesis of RNA and protein. That auxin-induced net synthesis of protein is repressed by actinomycin D indicates that auxin acts primarily to stimulate synthesis of RNA, as a result of which synthesis of protein is enhanced. The effect of kinetin alone on synthesis of RNA, or of kinetin on auxin-induced synthesis of RNA was variable, with either stimulation or inhibition observed in different experiments. Kinetin-enhancement of synthesis of both RNA and protein in subcellular fractions also varied, with enhancement of synthesis in 1 or all subcellular fractions among different experiments. The variable effect of kinetin did not seem to be related to the amount of endogenous or added auxin. The mode of action of kinetin is discussed.

Full text

PDF
1337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRELIN E. S. Mitosis in adult cartilage. Science. 1957 Apr 5;125(3249):650–650. doi: 10.1126/science.125.3249.650. [DOI] [PubMed] [Google Scholar]
  2. Fox J. E. Incorporation of a kinin, N, 6-benzyladenine into soluble RNA. Plant Physiol. 1966 Jan;41(1):75–82. doi: 10.1104/pp.41.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ku L. L., Romani R. J. Ribosomes from pear fruit. Science. 1966 Oct 21;154(3747):408–410. doi: 10.1126/science.154.3747.408. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Osborne D. J. Effect of Kinetin on Protein & Nucleic Acid Metabolism in Xanthium Leaves During Senescence. Plant Physiol. 1962 Sep;37(5):595–602. doi: 10.1104/pp.37.5.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUM E. L. Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science. 1961 Aug 25;134(3478):556–557. doi: 10.1126/science.134.3478.556. [DOI] [PubMed] [Google Scholar]
  7. Sacher J. A. Relationship between Auxin and Membrane-Integrity in Tissue Senescence and Abscission. Science. 1957 Jun 14;125(3259):1199–1200. doi: 10.1126/science.125.3259.1199. [DOI] [PubMed] [Google Scholar]
  8. Sacher J. A. Studies of permeability, RNA and protein turnover during ageing of fruit and leaf tissues. Symp Soc Exp Biol. 1967;21:269–303. [PubMed] [Google Scholar]
  9. Sacher J. A. Studies on Auxin-membrane Permeability Relations in Fruit and Leaf Tissues. Plant Physiol. 1959 Jul;34(4):365–372. doi: 10.1104/pp.34.4.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Skoog F., Armstrong D. J., Cherayil J. D., Hampel A. E., Bock R. M. Cytokinin activity: localization in transfer RNA preparations. Science. 1966 Dec 9;154(3754):1354–1356. doi: 10.1126/science.154.3754.1354. [DOI] [PubMed] [Google Scholar]
  11. TS'O P. O., SATO C. S. Synthesis of ribonucleic acid in plants. I. Distribution of ribonucleic acid and of protein among subcellular components of pea epicotyls. Exp Cell Res. 1959 May;17(2):227–236. doi: 10.1016/0014-4827(59)90214-9. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES