TABLE 5.

The borreliacidal activity of infected-mouse serum toward blood-phase and cultured B. burgdorferi is depleted by adsorption with DbpA

Antiserum or serumb	ELISA endpoint titer		Spirochetes surviving antiserum treatmenta
			Blood-phase spirochetes plus serum diluted:		Cultured spirochetes plus serum diluted:
	DbpA IgG	OspA IgG	1:50	1:250	1:50	1:250
Unadsorbed	1,024,000	<100	3	5	0	0
H. influenzae PAL-adsorbed	512,000	NDc	2	3.5	0	0
DbpA-adsorbed	32,000	ND	95	120	97	94
Uninfected-mouse serum	<500	ND	128	112	104	104

^a0.1 ml of cultured B. burgdorferi B31 diluted to approximately 10³/ml or 0.075 ml of plasma samples from spirochetemic C3H.SCID mice were incubated with antiserum at 33°C for 90 min and then plated on BSKII agarose for enumeration of viable spirochetes as CFU. Values are means of duplicate platings. 

^bAntisera pooled from C3H mice at week 16 of B. burgdorferi B31 infection was used untreated or after adsorption with PAL or DbpA. Uninfected-mouse serum was pooled from naive, uninfected C3H mice. 

^cND, not done.