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25× Dulbecco’s phosphate-buffered saline (DPBS)
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 3.75 M | 219.15 g |
| Na2HPO4 12H2O | 200 mM | 71.63 g |
| KCl | 67.5 mM | 5.03 g |
| KH2PO4 | 37.5 mM | 5.10 g |
| Double-distilled H2O | N/A | Fill up to 1 L |
| Total | 1 L |
Store at room temperature; stable for 1 year.
