Figure 2.

Chronic exercise training shift the hepatic metabolomic profile in mice fed high-fat diet and alters serum ACs and hydroxylated ACs

Heatmap showing a two-way hierarchical clustering of (A) serum, (B) liver, or (C) triceps, highlighting that ACs account for major shifts following exercise training. Total AC and AC-OH in (D) serum, (E) liver, and (F) muscle, (G) Short AC, (H) medium AC, and (I) long AC in serum, liver, and muscle.

(J) Chord visualization of significant, positive (chords represent r ≥ 0.5, p < 0.05) short ACs correlations demonstrate greater inter-tissue connectivity between muscle-liver (blue-to-red chords) and liver-serum (yellow-to-red chords), inter-tissue correlations. Conversely, exercise training reduced inter-tissue positive correlations (r ≥ 0.5, p < 0.05). of short AC.

(K) Chord representation (r ≥ 0.5, p < 0.05) of medium ACs correlations demonstrate greater inter-tissue connectivity between muscle-liver (blue-to-red chords) and many muscle intra-tissue correlations (more chords), while exercise training robustly reduced intra- and inter-tissue correlations (r ≥ 0.5, p < 0.05).

(L) Chord visualization (r ≥ 0.5, p < 0.05) shows a great degree of coordination between muscle-liver (blue-to-red chords) and intra-tissue liver and muscle correlations (more chords) while exercise training drastically reduced the intra- and inter-tissue correlations (r ≥ 0.5, p < 0.05).