TABLE 2.

T. cruzi EST matches to known sequences from trypanosomatids (not T. cruzi) and other organisms in NCBI databases^a

EST (TENS no.)b	Putative identificationc	Accession no.	BLASTd
Other trypanosomatids
1273	40S ribosomal protein L14	sp|P55842|	X
0051	40S ribosomal protein S12	sp|Q03253|	X
0057	40S ribosomal protein S14	sp|P19800|	X
1630	60S ribosomal protein L18	sp|P50885|	X
1451	60S ribosomal protein L30	sp|P49153|	X
1271	Activated protein kinase C receptor homolog mRNA	gb|U72205|	N
1408	Activated protein kinase C receptor homolog TRACK	gi|2952301|	X
0472	Cyclophilin A	gi|1532210|	X
1314	Cytochrome c oxidase polypeptide I	sp|P04371|	X
1285	Fructose-bisphosphate aldolase	pir||A54500 |	X
1354	GP63-3 surface protease homolog	gi|2196917 |	X
1942	GP63-3 surface protease homolog	gi|2196917	X
0362	H+-transporting ATPase (EC 3.6.1.35)	pir||A45598|	X
1233	Hypothetical protein 2	pir||A05123 |	X
1614	Intergenic region from the EF-1alpha upstream-associated gene-1 to the EF-1alpha gene	gb|U52680|	N
1421	Kinetoplastid membrane protein 11	gnl|PID|e225864	X
0020	mRNA for S12-like ribosomal protein	emb|Z15031|	N
1636	mRNA, clone Q14R1	emb|Z86119|	X
1943	Nucleic acid-binding protein	gi|1841864	X
0506	ORF 1	gnl|PID|e37082	X
1439	Phosphoglycerate kinase	sp|P41760|	X
1204	Phosphoglycerate kinase, glycosomal	sp|P41762|	X
0072	Probable 40S ribosomal protein S9	sp|P17959|	X
1291	Putative serine/threonine protein kinase	sp|Q08942|	X
0021	Ribosomal protein L27a	gb|U96757|	N
1345	Thioredoxin peroxidase	sp|Q26695|	X
Other organisms
1260	1,5-Heptosyltransferase I (Rfac) and Flax genes, complete Cds	gb|U40862|	N
0451	10-kDa heat shock protein, mitochondrial (Hsp10)	pir||S47532|	X
1352	14-3-3-Like protein	gi|1773328|	X
1290	2-Oxoglutarate dehydrogenase E1 component precursor	sp|P20967|	X
1838	3,2-trans-Enoyl-coenzyme A isomerase	sp|P42125|	X
1264	31.1-kDa protein In Dcm-Seru intergenic region	sp|P31658|	X
1485	40S ribosomal protein	sp|Q06559|	X
0047	40S ribosomal protein S10	sp|Q07254|	X
1750	40S ribosomal protein S13	sp|Q05761|	X
0904	40S ribosomal protein S15	sp|P20342|	X
0046	40S ribosomal protein S16	sp|P46294|	X
0084	40S ribosomal protein S17	sp|O01692|	X
0037	40S ribosomal protein S19	sp|P40978|	X
0012	40S ribosomal protein S2	sp|P25444|	X
1725	40S ribosomal protein S23	sp|P39028|	X
0063	40S ribosomal protein S25	sp|P46301|	X
0079	40S ribosomal protein S26e	sp|P21772|	X
0053	40S ribosomal protein S3	sp|Q06559|	X
0045	40S ribosomal protein S4	sp|P47961|	X
0038	40S ribosomal protein S6	sp|P02365|	X
0056	40S ribosomal protein Sa	sp|P38981|	X
0077	50S ribosomal protein L13	sp|O06260|	X
0949	55.2-kDa protein in Hxt8 5′ region	sp|P39976|	X
0028	60S ribosomal protein L10	sp|Q09127|	X
0027	60S ribosomal protein L11	sp|P42922|	X
0075	60S ribosomal protein L12	sp|P30050|	X
0954	60S ribosomal protein L13a	sp|P35427|	X
1482	60S ribosomal protein L17	sp|P24049|	X
1794	60S ribosomal protein L18a	sp|P41093|	X
0054	60S ribosomal protein L2	sp|P29766|	X
1589	60S ribosomal protein L21	sp|Q43291|	X
1003	60S ribosomal protein L22	sp|P13732|	X
1923	60S ribosomal protein L24	sp|P38663|	X
0049	60S ribosomal protein L26	sp|P47832|	X
0003	60S ribosomal protein L26-B	sp|P53221|	X
0008	60S ribosomal protein L3	sp|P35684|	N
0043	60S ribosomal protein L31	sp|P46290|	X
1875	60S ribosomal protein L32	sp|Q94460|	X
0081	60S ribosomal protein L35	sp|P42766|	X
0085	60S ribosomal protein L37a	sp|P32046|	X
0953	60S ribosomal protein L5	sp|Q26481|	X
0061	60S ribosomal protein L7	sp|P11874|	X
1925	60S ribosomal protein L7b	sp|P25457|	X
0033	60S ribosomal protein L9	sp|P49209|	X
1917	Acidic ribosomal protein P1	gi|2865615|	X
1468	Actin-interacting protein 2	sp|P46681|	X
1830	Acyl carrier protein	sp|P53665|	X
1801	Adenosylhomocysteinase	pir||A45569|	X
1946	ADP-ribosylation factor 1	sp|P35676|	X
0459	Af-9 Protein	sp|P42568|	X
1326	Alpha NAC/1.9.2. protein	gi|1142653|	X
1289	Alpha proteasome	gnl|PID|e321980	X
1374	Alpha-adaptin	gnl|PID|d1022258	X
1381	Alpha-enolase/tau-crystallin	gi|213085|	X
1520	Alpha-gliadin storage protein pseudogene	gb|U51305|	N
1944	TBP-interacting protein (TIP 49)	gnl|PID| d1029109	X
1301	Alternative oxidase	dbj||AB003176_1	X
1358	Arg kinase	prf||2020435A	X
1329	ATP synthase delta′ chain, mitochondrial precursor	sp|Q41000|	X
1582	ATP synthase F1 subunit alpha	gi|2258360|	X
1242	ATP-dependent RNA helicase, DEAD family (Dead)	gi|2648271|	X
1300	B0025.2 gene product	gi|1938574|	X
0281	BAC-146N21 chromosome X contains iduronate-2-sulfatase gene	gb|AC002315|	N
0265	BBC1 protein	gnl|PID|d1024629	X
1303	Bop1	gi|1679772|	X
1322	C25a1.6	gnl|PID|e275630	X
1635	CAGH26 mRNA	gb|U80739|	N
0644	Calmodulin	gi|167676|	X
0259	Caltractin	gb|U03270|	X
1184	Cctalpha chaperonin subunit	gi|2231589|	X
1281	Cell binding factor 2	sp|Q46105|	X
0416	Chaperonin containing T complex polypeptide 1, beta subunit; CCT-beta	gi|2559012|	X
1227	Chromosome 21q22.2 PAC clone P169K17, complete sequence	gb|AF015720|	N
1599	Cnjb	gi|161752|	X
1331	Contains similarity to enoly-coenzyme A hydratases	gi|2854202|	X
1592	Contains similarity to human spliceosome-associated protein	gi|2384908|	X
1862	Cyclophylin	gnl|PID|e267528	X
1294	Cytochrome b5	gi|2062405|	X
1856	Cytochrome P450-like TBP	gnl|PID|d1011583	X
1304	Cytoplasmic malate dehydrogenase	gi|2286153|	X
1272	Deoxyhypusine synthase mRNA	gb|U40579|	N
1435	Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (Pdc-E2)	sp|P08461|	X
1279	Dihyroorotate dehydrogenase	sp|P28272|	X
1851	DNA polymerase delta small subunit	gnl|PID|e243837	X
1376	DNA-directed DNA polymerase	pir||A55874|	X
1338	Dnaj protein	sp|P35515|	X
1293	Drome Pelota protein	sp|P48612|	X
1406	Dynein beta chain, flagellar outer arm	sp|Q39565|	X
1274	Enolase 1	P51555	X
1320	Enoyl-coenzyme A Hydratase, mitochondrial precursor	sp|P14604|	X
0438	Estb = esterase II	gb|S79600|	N
0501	Eukaryotic translation initiation factor 1a	sp|P38912|	X
1633	Excision repair protein Ercc-6	sp|Q03468|	X
1602	F21b7.26	gi|2809257|	X
1313	F421: this 421-aa ORF is 31% identical (3 gaps) to 91 residues of an approximately 864-aa protein, LOX3_SOYBN SW: P09186	gi|1787042|	X
1699	F44g4.1	gnl|PID|e236517	X
0581	Fast tropomyosin isoform	gi|2660868|	X
1284	G10 protein homolog	sp|P34313|
0002	Gene for putative ribosomal protein S31	emb|X14247|	N
0351	Genes for ORF1, ORF2, ORF3, ORF4, and Srb, partial and complete Cds	dbj|D64116|	N
1356	Glucosamine-6-phosphate isomerase	sp|P44538|	X
1722	Glycine cleavage system H protein precursor	sp|P23434|	X
1308	GTP-binding protein Ypt3	sp|P17610|	X
1400	Guanine nucleotide-binding protein alpha subunit	sp|P43151|	X
1327	H protein subunit of glycine decarboxylase mRNA, complete Cds	gb|AF022731|	X
1687	Heat shock protein 10	gi|2623879|	X
1493	Heat shock protein 75	gi|2865466|	X
0670	Heat shock protein HSLV	sp|P31059|	X
1437	Helicase	gi|780410|	X
0088	Histone H3	sp|P40285|	X
0094	Histone H4	gnl|PID|e324304	X
1192	Hit family protein 1	sp|Q04344|	X
0448	Homologous to acyl-coenzyme A dehydrogenase	gi|436861|	X
0421	Hydroproline-rich protein mRNA	gb|J03625|	X
1380	Hypothetical 20.8-kDa protein in Fgf-Vubi intergenic region	sp|P21286|	X
1341	Hypothetical 22.6-kDa protein F46c5.8 in chromosome Ii	sp|P52879|	X
1328	Hypothetical 23.5-kDa protein in Rfa2-Stb1 intergenic region	sp|P42844|	X
1910	Hypothetical 24.9-kDa protein in Sura-Hepa intergenic region	sp|P39219|	X
1330	Hypothetical 31.9-kDa protein in Gog5-Clg1 intergenic region	sp|P53081|	X
1302	Hypothetical 39.3-kDa protein in Gcn4-Wbp1 intergenic region	sp|P40004|	X
1364	Hypothetical 41.9-kDa protein in Sds3-Ths1 intergenic region	sp|P40506|	X
1177	Hypothetical 44.5-kDa protein in Pgpb-Pyrf intergenic region precursor	sp|P45576|	X
1824	Hypothetical 47.3-kDa protein in Ompx-Moeb	sp|P38821|	X
1585	Hypothetical 54.2-kDa protein in Cdc12-Orc6 intergenic region	sp|P38821|	X
0386	Hypothetical 90.8-kDa protein T05h10.7 in chromosome Ii	sp|Q10003|	X
1298	Hypothetical protein	gnl|PID|e326877	X
1385	Hypothetical protein	pir||S57550	X
1323	Hypothetical protein	gnl|PID|e339926	X
1618	Hypothetical protein	gnl|PID|e276614	X
1360	Hypothetical protein	gnl|PID|d1018647	X
1185	Hypothetical protein and to PIR:C48583 stress-inducible protein ST11	gi|1213541|	X
1186	Hypothetical protein YDR531w	pir||S69586	X
1812	Hypothetical protein YPL235w	pir||S61029	X
1476	Initiation factor 5a (Eif-5a) (Eif-4d)	sp|P56332|	X
1741	Insulinase	pir||SNHUIN	X
1369	Isocitrate dehydrogenase	gi|1277203|	X
1431	JC8.C	gnl|PID|e1247056	X
1580	KIAA0107-like protein	gi|2982297|	X
1805	Kiaa0305	gnl|PID|d1021601	X
1317	L1231-38	gi|2194152|	X
1315	L1231-6d	gi|2194149|	X
1609	L1439-18	gi|2266918|	X
1407	L4 protein (aa 1–256)	gi|4396(X17204)	X
0069	Large ribosomal subunit protein L13	sp|P38014|	X
1392	Male sterility 2-like protein	gnl|PID|e258459	X
1395	Meiotic spindle formation Protein Mei-1	sp|P34808|	X
0287	Mel-13a transcript	gb|U35309|	N
1889	Membrane-associated diazepam-binding inhibitor	prf||1911410A	X
1692	Mex-1	gi|1899062|	X
1399	Mitochondrial trifunctional enzyme beta subunit precursor	sp|Q60587|	X
1375	Mitotic centromere-associated kinesin	prf||2103270A	X
1515	mRNA for ribosomal protein L12	emb|X53504|	N
0018	mRNA for ribosomal protein S17	emb|X07257|	N
1443	mRNA for surface antigen P2	emb|X56810|	N
1336	No definition line found	gi|2384956|	X
1900	No definition line found	gi|2570931|	X
1391	Novel serine/threonine protein kinase	gnl|PID|d1006875	X
1335	N-terminal acetyltransferase complex Ard1 subunit homolog	sp|Q05885|	X
1941	NUC-1 negative regulatory protein PREG	sp|Q06712|	X
1505	Nucleoside diphosphate kinase	sp|P27950|	X
0667	Peptidase T (aminotripeptidase) (tripeptidase)	sp|P29745|	X
1311	Peptidylprolyl Isomerase	pir||S50141|	X
1905	Peroxisome targeting signal 2 receptor	gi|1907315|	X
1373	Phosphoglucomutase isoform 1 (glucose phosphomutase)	sp|P00949|	X
1347	Phosphoinositide-specific phospholipase C	prf||2123392A	X
1945	Phosphorylation regulatory protein HP-10	pir||A61382	X
1353	Phosphotyrosyl phosphatase activator	gi|974837|	X
1762	Potential Caax prenyl protease 1 (prenyl protein-specific endoprotease 1)	sp|Q10071|	X
0055	Probable 60S ribosomal protein L35	sp|P49180|	X
1370	Probable cell division control protein P55cdc	pir||A56021	X
1382	Probable membrane protein	pir||S51473	X
1412	Probable reductase protein	pir||A32950	X
1844	Proteasome iota chain (macropain iota chain)	sp|P34062|	X
1377	Proteasome subunit P112	gnl|PID|d1008506	X
1581	Protein kinase isolog	gi|2347199|	X
1359	Protein transport protein Sec61 alpha subunit	sp|P79088|	X
1393	Putative dimethyladenosine transferase	gi|2529685|	X
1390	Putative mevalonate kinase	sp|Q09780|	X
1371	Putative protein	gnl|PID|1253348	X
0016	Putative ribosomal protein L7A	gi|2529665|	X
1250	Pyruvate dehydrogenase E1 component, beta subunit precursor	sp|Q09171|	X
1947	RAS homolog GTPase rab28 isoform S	sp|P51157|	X
1948	RAS-related protein RAB-2	sp|Q05975|	X
0394	RAS-related protein Rab-23 (Rab-15)	sp|P35288|	N
1240	Red-1	gnl|PID|e209012	X
0062	Rer1 protein	sp|P25560|	X
1612	Ribonucleoprotein La	pir||A53781	X
0026	Ribosomal protein	gnl|PID|d1019682	X
0010	Ribosomal protein (Rp112)	gb|L04280|	N
0022	Ribosomal protein 15a (40S subunit)	emb|Z21673|	N
1882	Ribosomal protein L10, cytosolic	pir||JN0273	X
0065	Ribosomal protein L13.E, fruit fly	pir||S42877	X
0078	Ribosomal protein L15.E	sp|P30736|	X
0004	Ribosomal protein L3	sp|P39023|	X
1207	Ribosomal protein S11 homolog	pir||A48583	X
1526	Ribosomal protein S30	gnl|PID|e1173009	X
1332	SC2 = synaptic glycoprotein	pir||I56573	X
1318	Serine/threonine protein phosphatase 2b catalytic subunit, beta isoform	sp|P20651|	X
1297	Seryl-tRNA synthetase	pir||S71293	X
1758	Similar to acetyltransferases	gi|1825778|	X
1256	Similar to mammalian ZFP36 proteins in zinc finger regions	gi|1255428|	X
1819	Similar to pig tubulin-tyrosine ligase	gnl|PID|d1012156	X
1387	Similar to Saccharomyces cerevisiae BCS1 Protein, SWISS-PROT Accession no. P32839	dbj||D89136_1	X
1720	Similar to S. cerevisiae unknown, EMBL Accession no. Z68195	gnl|PID|d1014559	X
0319	Spermidine synthase mRNA	gnl|PID|e267359	X
1253	Succinate dehydrogenase	gnl|PID|e341165	X
1191	Succinyl coenzyme A synthetase alpha Subunit mRNA	gb|U23408|	N
1193	Succinyl-Coa ligase (Gdp-forming)	sp|P13086|	X
1278	Sulfated surface glycoprotein SSG185	prf||1604369	X
1397	Symbiosis-related protein	gi|2072023|	X
1684	T-complex protein 1, alpha subunit	sp|O15891|	X
1309	Thermostable carboxypeptidase 1	sp|P42663|	X
1288	Thyroid receptor-interacting protein 12	sp|Q14669|	X
1949	Translation initiation factor 5A	gnl|PID|e266087	X
1416	Triacylglycerol lipase	sp|P21811|	X
1368	Ubiquinol--cytochrome C reductase	pir||A44033	X
1389	UDP-glucose 4-epimerase (Gale-2)	gi|2648515|	X
1405	Unknown	gnl|PID|e223630	X
1436	Vacuolar aminopeptidase I precursor	gi|699234|	X
1307	Wd40 repeat protein 2	sp|P54686|	X
1182	Weak similarity to SP:YAD5_CLOAB (P33746) hypothetical protein and to PIR:C48583 stress-inducible protein STI1	gi|1213541	X
1325	White	gi|2182784	X
0449	Yeast probable phosphatidylinositol-4-phosphate 5-kinase	sp|P34756|	X
1324	ZK795.D	gnl|PID|e1188511	X

^aAll significant similarities (P ≤ 10⁻⁵) of nonredundant ESTs against non-T. cruzi entries in NCBI nonredundant databases are listed, together with the accession numbers and the program used for the search. Matches are sorted according to the “Other trypanosomatids” and “Other organisms” categories. A complete (including matches to T. cruzi) and more detailed table is available at http://www.iib.unsam.edu.ar/genomelab/tcruzi/5ests.html. 

^bEST names in the dbEST are the four-digit numbers given here preceded by TENS. 

^cORF, open reading frame; aa, amino acids. 

^dN, BLASTn; X, BLASTx.