Table 4.
Innate immune cell panel used for baseline immunophenotyping
| Channel | Fluorochrome | Antibody | Clone | Dilution | Company | Catalog # |
|---|---|---|---|---|---|---|
| B515 | BB515 | CD11b | M1/70 | 1:200 | BD | 564455 |
| B710 | PerCP-Cy5.5 | Ly-6C | HK1.4 | 1:150 | Invitrogen | 45-5932-80 |
| R660 | APC | CD40 | HM40-3 | 1:160 | Invitrogen | 17-0402-82 |
| R780 | APC-eFluor780 | CD8α | 53–6.7 | 1:100 | Invitrogen | 47-0081-80 |
| G575 | PE | CD86 | PO3.1 | 1:160 | Invitrogen | 12-0861-81 |
| G610 | PE/Dazzle594 | F4/80 | BM8 | 1:200 | Biolegend | 123145 |
| G660 | PE-Cy5 | CD80 | 16-10A1 | 1:200 | Invitrogen | 15-0801-81 |
| V450 | eFluor450 | MHC Class II | M5/114.15.2 | 1:800 | Invitrogen | 48-5321-80 |
| V510 | Aqua | Live/dead | 1:1000 | Invitrogen | L34966 | |
| V610 | BV605 | CD4 | RM4-5 | 1:400 | Biolegend | 100548 |
| V710 | BV711 | NKp46 | 29A1.4 | 1:100 | Biolegend | 137621 |
| U396 | BUV395 | Ly-6G | 1A8 | 1:50 | BD | 563978 |
| U500 | BUV496 | CD11c | N418 | 1:133 | BD | 750450 |
| U570 | BUV563 | CD3 | 145-2C11 | 1:200 | BD | 749277 |
| U660 | BUV661 | CD19 | 1D3 | 1:50 | BD | 612971 |