Fig. 7.

The inactivation of p38-MAPK/HIF-1α/p53 signaling axis is triggered by MSN@Res and its role in regulation of fibroblast autophagy and apoptosis.

(A),(B)and(C) The HSFs were pretreated with 10 μM SB203580 for 2 h prior to 100 μM MSNs@Res treatment for 48 h with or without 25 μg/mL anisomycin, and then the transition from autophagy to apoptosis in HSFs was determined by immunofluorescence analysis, where as green fluorescence represents Tunnel-positive cells, indicating apoptosis, and red fluorescence represents LC3-II-positive cells, indicating autophagy (n = 3, compared with control，^△△P< 0.01，^△△△△P < 0.0001；compared with MSN@Res， ^∗∗∗P < 0.0001， ****P < 0.0001；compared with MSN@Res+S, ^##P < 0.01，^####P < 0.0001). (D),(E)and(F) Western blot analysis of HIF-1α, p53, autophagy and apoptosis-related proteins expression. (n = 3, compared with MSN@Res , **P < 0.01, *P < 0.05; compared with MSN@Res + S , ^△P < 0.05, ^△△P < 0.01,^△△△P < 0.001) Note: M: MSN@Res, A: Anisomycin, S: SB203580. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)