TABLE 1.
Traits of Streptococcus pyogenes strain AB1a pathogenic gene families that were not present in serotype M1 SF370,b identical to pathogenic streptococcus
| Data from PathogenFinder ver. 1.1c | Data from DFASTe and BLASTpf | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Pathogenic streptococcus d | Accession no. (Protein no.) | Product | Contig no. | Nucleotide start | Nucleotide end | Size (bp) | Product | Product | |
| 1 | S. pyogenes MGAS9429 | CP000259.1 (ABF31304.1) | Transcriptional regulator, AraC family | 12 | 38,071 (reverse) | 36,866 | 1,206 | YSIRK-targeted surface antigen transcriptional regulator | Bacterial regulatory helix-turn-helix, AraC family protein |
| 2 2 |
S. dysgalactiae subsp. equisimilis GGS_124 | AP010935.1 (BAH82621.1) | DNA integration/recombination/inversion protein | 1 | 35,315 | 36,478 | 1,164 | Site-specific integrase | Site-specific integrase |
| 3 | S. pyogenes MGAS9429 | CP000259.1https://www.ncbi.nlm.nih.gov/nuccore/CP000017.2/(ABF31725.1) | Phage protein | 4 | 315,992 | 316,633 | 642 | Hypothetical protein | Hypothetical protein |
| 4 | S. pyogenes MGAS6180 | CP000056.2 (AAX72126.1) | Phage protein | 4 | 311,016 (reverse) | 310,534 | 483 | Hypothetical protein | DUF669 domain-containing protein in phage proteins |
| 5 | S. pyogenes MGAS10394 | CP000003.1 (AAT87334.1) | Holin | 4 | 280,566 (reverse) | 280,111 | 456 | Hypothetical protein | Phage holin family protein |
| 6 | S. pyogenes MGAS9429 | CP000259.1https://www.ncbi.nlm.nih.gov/nuccore/CP000017.2/(ABF31767.1) | Phage protein | 4 | 293,210 (reverse) | 292,785 | 426 | Hypothetical protein | Hypothetical protein |
| 7 | S. pyogenes MGAS6180 | CP000056.2 (AAX72112.1) | Phage protein | 4 | 301,081 | 301,458 | 378 | Type II toxin-antitoxin system HicB family antitoxin | Type II toxin-antitoxin system HicB family antitoxin |
| 8 | S. pyogenes MGAS6180 | CP000056.2 (AAX72904.1) | Phage protein | 27 | 22 | 429 | 408 | Hypothetical protein | Hypothetical protein |
| 9 | S. pyogenes MGAS8232 | AE009949.1 (AAL97901.1) | Hypothetical phage protein | 4 | 313,944 (reverse) | 313,615 | 330 | Hypothetical protein | Hypothetical protein |
| 10 | S. pyogenes MGAS10394 | CP000003.1 (AAT87160.1) | Unknown phage protein | 4 | 318,467 | 318,772 | 306 | Hypothetical protein | Membrane protein |
| 11 | S. pyogenes MGAS8232 | AE009949.1 (AAL97899.1) | Hypothetical phage protein | 4 | 313,424 (reverse) | 313,140 | 285 | Hypothetical protein | Hypothetical protein |
| 12 | S. pyogenes MGAS10394 | CP000003.1 (AAT87144.1) | Unknown phage protein | 4 | 297,702 (reverse) | 297,478 | 225 | Hypothetical protein | Hypothetical protein |
| 13 | S. pyogenes NZ131 | CP000829.1 (ACI60688.1) | Hypothetical protein | 1 | 26,121 (reverse) | 25,978 | 144 | Hypothetical protein | Hypothetical protein |
| 14 | S. pyogenes MGAS10270 | CP000260.1 (ABF33634.1) | Phage protein | 4 | 300,844 | 301,029 | 186 | Hypothetical protein | Toxin-antitoxin system, toxin component, HicA family |
| 15 | S. pyogenes MGAS10394 | CP000003.1 (AAT87133.1) | Unknown phage protein | 4 | 291,758 (reverse) | 291,585 | 174 | Hypothetical protein | Hypothetical protein |
| 16 | S. pyogenes MGAS10394 | CP000003.1 (AAT87145.1) | Unknown phage protein | 4 | 297,865 (reverse) | 297,695 | 171 | Hypothetical protein | Hypothetical protein |
GenBank nucleotide accession number BTGW00000000.1. GenBank assembly accession number GCF_033008475.1.
GenBank nucleotide accession number AE004092.2. GenBank assembly accession number GCF_000006785.2.
The PathogenFinder ver. 1.1 (https://cge.cbs.dtu.dk/services/PathogenFinder/, Center for Genomic Epidemiology) was applied to obtain an overview of the genomic pathogenic gene families.
We found the data regarding the pathogenic streptococcus, GenBank accession number, and protein number using the PathogenFinder ver. 1.1.
The DDBJ Fast Annotation and Submission Tool (DFAST; https://dfast.nig.ac.jp/) was used to confirm the product (coding DNA sequence) obtained using PathogenFinder ver. 1.1.
The Protein Basic Local Alignment Search Tool (BLASTp; https://blast.ncbi.nlm.nih.gov/Blast.cgi) of the National Center for Biotechnology Information was also applied to confirm the product (coding DNA sequence) obtained using PathogenFinder ver. 1.1.