Skip to main content
. 2023 Sep 27;208(11):1177–1195. doi: 10.1164/rccm.202305-0924OC

Figure 1.

Figure 1.

Figure 1.

CD8+ T effector memory CD45RA+ (TEMRA) and T resident memory (TRM) cells have increased abundance in mild-moderate chronic obstructive pulmonary disease (COPD) lung. (A) Study approach. Lung tissues surgically resected from patients without chronic lung disease (control), mild-moderate COPD, or emphysema without airway obstruction (emphysema w/o obsx) were compared with lungs from explanted “donors” and patients undergoing lung transplantation for COPD (end-stage COPD). Single-cell transcriptomic and proteomic analyses of these lung tissues were compared with independent patient cohorts and a murine model of COPD. (B–D) Single-cell RNA-sequencing (scRNA-seq) and scCITE-seq analysis of lung tissue. (B) UMAP visualization of cell clusters; inset: CD8+ T-cell clusters. (C) Transcriptional and protein markers defining CD8+ T-cell clusters. Red indicates cell clusters enriched in mild-moderate COPD compared to other patient subcohorts. (D) Relative abundance of cell clusters in pairwise comparison of patient subcohorts using the scCODA computational pipeline; red indicates cell clusters enriched in the red subcohort compared with control or donor. (E) Relative abundance of cell clusters in mild-moderate COPD tested by the MiloR computational pipeline; red indicates clusters enriched compared with control. AT2 = alveolar type II; CITE-seq = cellular indexing of transcriptomes and epitopes; NK = natural killer.