Figure 2.

CD8⁺KLRG1⁺ T effector memory CD45RA⁺ (TEMRA) and T resident memory 1 (T_RM1) cells have distinct immunophenotypes in mild-moderate chronic obstructive pulmonary disease (COPD) lung. CD8⁺ T-cell clusters are examined in the multiomic integration of single-cell RNA-seq, CITE-seq, and T-cell receptor (TCR) sequence repertoire analysis of lung tissue. (A–E) Lung tissue from mild-moderate COPD is examined. (A and B) CITE-seq dataset of protein expression. (A) Heatmap of proteins differentially expressed in CD8⁺KLRG1⁺ TEMRA and T_RM1 cells, by CD8⁺ T cell cluster. (B) UMAP visualization of differentially expressed proteins. (C–E) RNA-seq dataset of mRNA expression. (C) Heatmap of transcripts differentially expressed in CD8⁺KLRG1⁺ TEMRA and T_RM1 cells, by CD8⁺ T cell cluster. (D) UMAP visualization of differentially expressed genes. (E) UMAP visualization of differentially expressed pathways and associated genes. (F) Boxplot of clonotype diversity measured by Shannon index by patient (dot) and grouped by subcohort. (G) The sharing of TCR clonotype among CD8⁺ T cell clusters in mild-moderate COPD lungs. (H and I) Each CD8⁺ T cell is classified by its clonotypic expansion, ranging from single T cell with a specific clonotype to hyperexpanded (>100 T cells with same TCR clonotype). The proportion of CD8⁺ T cells in each classification is shown by: (H) patient subcohort; and (I) CD8⁺ T-cell cluster within mild-moderate COPD lung tissue. Emphysema w/o obsx = emphysema without airway obstruction; NK = natural killer.