Figure 4.

CD8⁺KLRG1⁺ T cells have increased abundance in mild-moderate chronic obstructive pulmonary disease (COPD) lung compared with end-stage COPD. Single-cell RNA-sequencing (scRNA-seq) datasets from this study and Adams and colleagues (14) were integrated. (A) UMAP visualization of cell clusters with lineage indicated by color. Green dotted line denotes natural killer (NK) and T-cell clusters. (B) Relative abundance of lymphoid clusters, by patient subcohort. *Indicates CD8⁺ T1 (tan) and CD8⁺KLRG1⁺ (orange) T cells. (C) Relative abundance of cell clusters in pairwise comparison of patient subcohorts using the scCODA computational pipeline; red indicates cell clusters enriched in COPD compared with their respective control. Red text highlight CD8⁺KLRG1⁺ and T1-cell clusters. (D) On the left, CD8⁺ T cells in the scRNA-seq–only analysis (from Figure 4A) are mapped to themselves on the right in the multiomic (scRNA-seq/CITE-seq) analysis (from Figure 1B). The cell cluster annotations for either the RNA-seq only (left) or multiomic (right) analyses are shown. Cells in the KLRG1⁺ T effector memory CD45RA⁺ (TEMRA) and T resident memory 1 (T_RM1) multiomic clusters are highlighted as pink or green, respectively.