Table 3.
Clinical characteristics of study patients (n=653).
| Characteristics1 | mNGS detection2 | P value3 | |
|---|---|---|---|
| mNGS positve (n=472) | mNGS negative (n=181) | ||
| Age(years), Median (IQR)4 | 57(39·75-69) | 53·5(33-66) | 0·073 |
| Pediatric patients, n (%)5 | 38(8·1) | 16(8·8) | 0·734 |
| Gender(male), n (%) | 280(59·3) | 87(48·1) | 0·009 |
| Immunocompromised, n (%) | 341(72·2) | 108(59·7) | 0·002 |
| Intensive care unit, n (%) | 100(21·2) | 22(12·2) | 0·008 |
| Underlying condtion | |||
| Haematological disease, n (%) | 134(28·4) | 38(21) | 0·055 |
| FUO, n (%) | 94(19·9) | 56(30·9) | 0·003 |
| COPD, n (%) | 63(13·3) | 20(11) | 0·430 |
| Solid cancer, n (%) | 36(7·6) | 6(3·3) | 0·044 |
| Cerebrovascular disease, n (%) | 33(7) | 8(4·4) | 0·225 |
| Serious trauma/surgery, n (%) | 24(5·1) | 8(4·4) | 0·725 |
| Immunodeficiency diseases, n (%) | 16(3·4) | 8(4·4) | 0·531 |
| IAI, n (%) | 15(3·2) | 7(3·9) | 0·662 |
| Cirrhosis, n (%) | 9(1·9) | 8(4·4) | 0·071 |
| CHD, n (%) | 8(1·7) | 5(2·8) | 0·382 |
| GI bleeding/perforation, n (%) | 7(1·5) | 2(1·1) | 1 |
| Sepsis, n (%) | 6(1·3) | 0(0) | 0·287 |
| UTI, n (%) | 6(1·3) | 1(0·6) | 0·709 |
| Diabetes, n (%) | 5(1·1) | 2(1·1) | 1 |
| Meningitis, n (%) | 4(0·8) | 6(3·3) | 0·052 |
| CKD, n (%) | 4(0·8) | 1(0·6) | 1 |
| Other, n (%) | 8(1·7) | 5(2·8) | 0·575 |
mNGS, metagenomic next-generation sequencing; IQR, interquartile range; FUO, fever of unknown origin; COPD, chronic obstructive pulmonary disease; IAI, intra-abdominal infection; CHD, coronary heart disease; GI, gastrointestinal; UTI, urinary tract infection; CKD, chronic kidney disease.
1. For multiple diseases, calculated according to primary disease; 2. mNGS positve is the pathogen fragment reported by sequencing, which may include true positive/false positive for final clinical interpretation. And mNGS positive includes possible bloodstream infection and other system infections; 3. Age P values assess by student t test, and other patient characteristic P values assess by Pearson Chi-Square test or Fisher’s exact test 4. IQR refers to the calculated quartile of 0·25 and 0·75; 5. Pediatric patients refer to age ≤14 years old.