Table 5.
Comparison of distributions of sequencing reads for clinically relevant and irrelevant organisms (lines indicate median values) .
| Microorganisms detected by mNGS (n=735) | Reads Range | Median Reads(IQR) | P 1 |
|---|---|---|---|
| Bacteria (n=380) | |||
| Clinically relevant(n=274) | 3-57969 | 98 (7,23) | < 0·01 |
| Clinically irrelevant(n=106) | 3-36 | 5(3,11) | |
| Virus(n=235) | |||
| Clinically relevant2(n=71) | 3-41266 | 69(20,283) | 0·03 |
| Clinically irrelevant(n=164) | 3-51 | 8(4,16) | |
| Fungus(n=107) | |||
| Clinically relevant(n=61) | 3-4454 | 102(32,293) | < 0·01 |
| Clinically irrelevant(n=46) | 3-31 | 6(3,10) | |
mNGS, metagenomic next-generation sequencing; IQR, interquartile range; GNB, Gram-negative bacteria; GPB, Gram-positive bacteria.
1. P < 0·05 indicates a significant correlation; 2. Clinically relevant means that the final clinical diagnosis is the causative pathogen, and some viruses (such as EBV) are true positive but the clinical significance of infection is not clear, and it is judged as clinically irrelevant.